Simplifying CRISPR

eveloping cell lines for use as accurate models for disease has always been a challenging task. In order to replicate phenotypes or enable downstream experimentation, genome engineering of cell lines is required. Techniques enabling genome modification have evolved over a number of years, from simple viral vectors used to insert sequences in random locations, to more sophisticated methods that enable targeting of specific loci. The ability to edit genes in human cells in a highly targeted manner has been transformative, expanding our capacity to understand human genetics and providing the potential for therapeutic applications.